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Bioflim probes

CDy11 - Amyloid targeting [Patent pending]

■ Chemical structure & Information
Bacterial biofilm is a self-generated extracellular polymeric substance (EPS), composed of extracellular DNA, polysaccharides, and proteins such as amyloids. CDy11 (Compound of Designation yellow 11) is discovered through a screening for bacteria with functional amyloids in Pseudomonas aeruginosa (Fap).
■ Cell Image
Evaluation of target of CDy11. (A) Transmission electron microscopic image of purified amyloid. Scale bar, 400 nm. (B) Surface plasma resonance assay with CDy11 and purified amyloid after immobilization on chips. Values were calculated by measurements for their response unit by flowing ThT and CDy11. (C) Co-localization of CDy11 and Fap antibody. Biofilms were incubated with primary Fap antibody and secondary antibody linked with Cy5. Subsequently, CDy11 was treated for 1 h before acquiring images. The images were captured using a fluorescence microscope equipped with ×100 objective lens. Scale bars, 10 μm. The images are shown in pseudocolors.
■ Related probes: CDy14, CDr15
■ Known Property
Bacterial amyloids probe
■ Application
Immunofluorescence
■ Cell selectivity mechanism
POLD (Bacterial amyloids)
■ Reference
1.
Detection of pathogenic biofilms with bacterial amyloid targeting fluorescent probe, CDy11, Kim, J. Y.; Sahu, S.; Yau, Y. H.; Wang, X.; Shochat, S. G.; Nielsen, P. H.; Dueholm, M. S.; Otzen, D. E.; Lee, J.; Delos Santos, M. M.; Yam, J. K.; Kang, N. Y.; Park, S. J.; Kwon, H.; Seviour, T. W.; Yang, L.; Givskov, M.; Chang, Y. T.* J. Am. Chem. Soc. 2016, 138, 402-407.
2.
A mouse ear skin model to study the dynamics of innate immune responses against Staphylococcus aureus biofilms, Abdul Hamid, A. I.; Nakusi, L.; Givskov, M.; Chang, Y. T.; Marquès, C.; Gueirard, P. BMC Microbiol. 2020, 20:22.

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■ Storage
① Delivery: Room Temperature
② Dried compound: 4°C or -20°C
③ Compound solution: 4°C or -20°C

■ General Use Guide
•
More than 1/100 dilution of 10mM of DMSO stock solution is essential
•
For biomedical use to avoid DMSO concentration higher than 1%.
•
Working concentrations for specific applications should be determined by the investigator.
•
It is recommended to use up the buffer diluted solution within one day. The compound may be decomposed or
precipitated out from buffer solution.
■ Data sheet PDF file
CDy11-data-sheet-v0.1-2.pdf660.46KB
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