Home
🔬PROBES
FAQ
Subscribe

Histamine sensor

Histamine Blue [Catalog # P060]

■ Chemical structure & Information
Histamine Blue is a mesoionic acid fluoride showing a 14-fold fluorescence increase upon condensation with histamine. Histamine Blue displays very good selectivity over a broad range of signalling molecules and metabolites due to the catalytic effect of the imidazole group, and is the first fluorescent small molecule for imaging histamine in live RBL-2H3 basophils. Histamine Blue can also monitor the uptake and de novo synthesis of histamine in live RAW 264.7 macrophages and its fluorescence intensity correlates well with the histamine levels in cell extracts.
■ Cell Image
A) Reaction of 4a with histamine in PBS. Fluorescence spectra of 4a before (black) and after (blue) reaction with histamine; exc: 340 nm. Fluorescent images of PBS solutions of 4a and 5i under a 365 nm lamp.
B) RBL-2H3 basophils after incubation with 20µM Histamine Blue. (a) Bright field image, (b) DRAQ5 nuclear counterstaining (TXR), (c) Histamine Blue staining (DAPI), (d) merged a, b and c. Scale bar: 20 mm.
Three-color imaging for granulocytes. a) Schematic of granulocytes sorted by Fluorescence-Activated Cell Sorting (FACS). b) Three-color imaging of granulocytes. The sorted granulocytes were stained NeutropG (0.2 μM), Histamine Blue (10 μM), and APC anti-Siglec-8 for 1 h.
■ Known Property
Basophil selective through histamine sensing
■ Application
Immunofluorescence
■ Cell selectivity mechanism
Unknown
■ Reference
1.
Imaging Histamine in Live Basophils and Macrophages with a Fluorescent Mesoionic Acid Fluoride, Kielland, N.; Vendrell, M.; Lavilla, R.; Chang, Y. T. Chem. Commun. 2012, 48, 7401-7403
2.
Neutrophil Selective Fluorescent Probe Development through Metabolism-Oriented Live-cell Distinction, Gao, M.; Lee, S. H.; Park, S. H.; Ciaramicoli, L. M.; Kwon, H. Y.; Cho, H.; Jeong, J.; Chang, Y. T.* Angew. Chem. Int. Ed. Engl. 2021, 60, 23742-23749.

■ ORDER
International
USD 350 + VAT (10%) + Fedex delivery charge
In Korea
KRW 450,000 + VAT (10%)
100 nmol (dried form: equvalent to 1 mM / 100 µL)

■ Storage
① Delivery: Room Temperature
② Dried compound: 4°C or -20°C
③ Compound solution: 4°C or -20°C

■ General Use Guide
•
More than 1/100 dilution of 10mM of DMSO stock solution is essential
•
For biomedical use to avoid DMSO concentration higher than 1%.
•
Working concentrations for specific applications should be determined by the investigator.
•
It is recommended to use up the buffer diluted solution within one day. The compound may be decomposed or
precipitated out from buffer solution.
■ Data sheet PDF file
Histamine blue data sheet.pdf467.10KB
Made with Slashpage