AGN (Advanced Glycation end-products Negative) is a negative control probe designed to validate the selectivity of AGO, engineered to prevent AGEs binding and produce minimal fluorescence. AGN features a meta-methoxybenzyl substituent, which prevents interactions with Schiff bases and Amadori products, making it an ideal negative control. It shares the same fluorescence properties as AGO (Excitation 490 nm / Emission 570 nm) but does not emit significant fluorescence in the presence of AGEs. By comparing AGO and AGN staining, AGN helps distinguish true AGEs-specific fluorescence from nonspecific background signals, ensuring reliable AGEs detection.
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AGO selectivity to AGEs derived from the collagen model. The collagen model was utilized to confirm the selectivity of AGO. The fabricated collagen was incubated with 500 mM ribose for 1 week to form the glycation. The collagen hydrogel was stained using an anti-collagen I antibody, and then stained using (A) AGN (negative control) and (B) AGO.
Development of a specific fluorescent probe to detect advanced glycation end products (AGEs), Cho, H.; Hong, N.-K.; Yong, I.; Kwon, H.-Y.; Kang, N.-Y.; Ciaramicoli, L. M.; Kim, P.; Chang, Y.-T.* J. Mater. Chem. B 2024, 12, 6155–6163.
