AGO (Advanced Glycation end-products Orange) is an AGE-selective probe with higher selectivity and sensitivity than conventional methods, allowing effective visualization of AGEs accumulation in a collagen ECM model. It has a BODIPY-based structure with a meta-phenoxybenzyl substituent and exhibits fluorescence at Excitation 490 nm / Emission 570 nm. AGO is designed for in vitro use, specifically for detecting AGEs in extracellular matrix (ECM) models. The meta-phenoxybenzyl moiety facilitates non-covalent interactions with Schiff bases and Amadori products, ensuring AGEs-specific binding. AGO has been validated through in vitro glycation models, including BSA-Ribose and collagen ECM systems, demonstrating its strong selectivity for AGEs.
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AGO selectivity to AGEs derived from the collagen model. The collagen model was utilized to confirm the selectivity of AGO. The fabricated collagen was incubated with 500 mM ribose for 1 week to form the glycation. The collagen hydrogel was stained using an anti-collagen I antibody, and then stained using (A) AGN (negative control) and (B) AGO.
Development of a specific fluorescent probe to detect advanced glycation end products (AGEs), Cho, H.; Hong, N.-K.; Yong, I.; Kwon, H.-Y.; Kang, N.-Y.; Ciaramicoli, L. M.; Kim, P.; Chang, Y.-T.* J. Mater. Chem. B 2024, 12, 6155–6163.
